Int J Food Sci Nutr. Gekko, K. (1971). ; Prasad, D.H.L. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Keywords: Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. (2008). Ubbelohde, Leo. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Food Funct. Springer Nature. Chemical penetration enhancers for transdermal drug delivery systems. Porcine wound models for skin substitution and burn treatment. 2012;29(7):201729. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Epub 2021 Feb 9. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Gold MH, Biron JA. What is dextran? Application areas - TdB Labs AB Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). 2013;3(5):3588605. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. (1998). These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). The micropores of the human cadaver skin were regenerated according to the time. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. government site. Small Intestinal Permeability and Gut-Transit Time Determined TEWL measurements were taken 30min after microporation of the human and pig skin. Progress in Polymer Science, 30, 38-70. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. ; Bos, G.W. 2023 BioMed Central Ltd unless otherwise stated. Polymers | Free Full-Text | Confinement-Induced Fractionation and HSE and DK helped to porate the skin and to measure TEWL. ; Dutt, N.V.K. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. The human skins were from the back or thigh of a 24-year-old woman. Carbohydrate Polymers 87, 405-410. dextran Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Carbohydrate Polymers, 39, 377-380. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Dextran 670 GPC Standard | Pharmacosmos Dextran Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. ; Wood, P.J. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. ; Picout, D.R. Garcia de la Torre, J.; Carrasco, B. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. hydrodynamic Regulating dynamic signaling between hematopoietic stem cells Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. In addition, we evaluated the regeneration of SC after microporation. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. (2008). where tr is the column retention time in minutes (2 of 0.9792). Advances in Carbohydrate Analysis, 1, 63-144. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. The mechanism of the dextran-induced red blood cell Aggregation. Appl Biochem Biotechnol. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Not for use in diagnostic procedures. PMC Amersham Biosciences, AA Edition, www.amershambiosciences.com. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Morris, G.A. of different origins. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). 2012;87(6):1728. 2011;16(2):518. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Structural and biocompatibility properties of dextran from Expert Rev Med Devices. 2000;3(9):31826. Pure Appl. Study of temperature influence. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Bohdanecky, Miloslav (1996). Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. ; Ghosh, T.K. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Carbohydr Polym. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Journal of Physical Chemistry. Solution properties of branched dextran. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Elias, Hans-Georg (1975). In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Bahary, W.S. California Privacy Statement, The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Biomaterials. Cell permeabilization using a non-thermal plasma - IOPscience Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Degrees of long-chain branching in dextrans. Banga AK. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. ; Hogan, M.P. ; Fran, H.P. The micropore formed at the SC through to the epidermis, enabling drug delivery. This study was supported by the LG Electronics. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). Environ Toxicol Pharmacol. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. They concluded that a power law behavior seems to be approached only in the small molecular weight region. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. CAS Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Stokes radius J Appl Toxicol. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. In fact, short chains do contain minor branching points. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Eremeeva, T. E.; Bykova, T. O. Determination of intrinsic viscosities of macromolecules and nanoparticles. Keywords: FITC dextran; intestinal permeability; transit time. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). (2013). Springer Editions. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. 2006;3(3):37385. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. (2003). Polyelectrolytes as secondary calibration standards for aqueous SEC. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. American Journal of Food Science and Technology 2013, 1(3), 60-66. Epub 2012 Oct 18. principles of dynamic light scattering ; Grishchenko, A.E. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Fluorescent and Biotinylated DextransSection 14.5 Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th official website and that any information you provide is encrypted This conjecture found to be correct. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. Dextran. Ahn, G.Y., Eo, HS., Kim, D. et al. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Soc., 21, 1, 78-86. Terms and Conditions, Intrinsic viscosity-molecular weight relationship for chitosan. (1990). 8. 8580 Macromolecules 34, Notes - NIST what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. Walkenhorst, S.; Olivier, R. (2003). Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Would you like email updates of new search results? Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Increased epidermal laser fluence through simultaneous ultrasonic microporation. National Library of Medicine Carbohydrate Polymers, 74, 372-378. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Journal of Chemical Education, 80(9), 1036-1038. FITC-dextran solution (600L) was added to the donor chamber. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Dextrans can function efficiently as anterograde or retrograde tracers, Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). (1958). Carbohydrate Polymers 72, 356-360. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. 113, 81-86. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. The above data are not useful because it can not be compared with those obtained in this work. Table 2 compares the properties of nanoparticles coated with one or other polymer. Viscosity of Liquids. By using this website, you agree to our Format), Citation-(BibTeX
SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. 2012;101(8):28619. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. These studies were carried out at the Hisko (Gunpo, Korea). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. 2010;4(1):117. The slope is a value and intercept is equal to the ln kMH value [47]. Soc., 16, 3A, 426-433. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. ; Patel, T.R. Intrinsic viscosity of bovine serum albumin conformers. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. 7 8 For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. 2016;47:15964. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in J Control Release. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. Chem. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Webdextran hydrodynamic radius. Hydrodynamic Radius Corp. (Seoul, Korea). Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). The receptor chamber was filled with PBS. Macromolecules. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Biophysical Journal, 87, 4259-4270. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Smith, David R.; Raymonda, John W. (1972). 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Journal of Controlled Release, 119, 5-24. Sachdeva V, Banga AK. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Data File Dextran. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. A single frequency laser is directed to the sample contained in a cuvette. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. 2009;8(2):1739. Skin barrier disruption by acetone: observations in a hairless mouse skin model. Pavlov, G.M. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids.